NFixPlanet

Detect and quantify nitrogen-fixing microorganisms (diazotrophs) from genomes and metagenomes.

NFixPlanet combines profile Hidden Markov Model (HMM) annotation, genomic context validation, and coverage-based abundance estimation using a curated diazotroph reference database.

Available databases & workflows
Available on Zenodo
NFixPlanet Database

Full NFixPlanet resource collection including the gene database, metagenome database, genome archive, HMM profiles, and associated metadata.

Available here
annotate Genome Annotation

Identifies nitrogen fixation genes and operons in genome assemblies, MAGs, or contigs using HMMs and genomic context filtering.

profile Metagenome Quantification

Maps short reads to a diazotroph reference database and computes gene, genome, and taxonomic relative abundance. Available via pip / conda.

Run nfixplanet annotate

Accepted format: .fasta.gz — max 10 MB

The job is executed on CLoWM. Results are stored for download once the workflow completes.

How to use this tool
  1. Upload a gzip-compressed FASTA file (.fasta.gz, max 10 MB).
  2. The annotate workflow is submitted to CLoWM and runs automatically in the cloud.
  3. You are redirected to a results page where you can track progress.
  4. Once complete, annotated output files are available for download.

Need the full tool locally? Install via pip or bioconda:

conda install -c bioconda nfixplanet